| 1 | –Dock Boggs | Country Blues |
| 2 | –Washington White | The Panama Limited |
| 3 | –Amédée Ardoin And Dennis McGee | Les Blues De Voyage (Travel Blues) |
| 4 | –Burnett And Rutherford | Ladies On The Steamboat |
| 5 | –Sleepy John Estes | Someday Baby Blues |
| 6 | –Hoyt Ming And His Pep Steppers | Indian War Whoop |
| 7 | –Frank Stokes | 'Tain't Nobody Business If I Do (Part 1) |
| 8 | –Dixieland Jug Blowers | Banjoreno |
| 9 | –A.C. (Eck) Robertson | Sallie Gooden |
| 10 | –Mike Hanapi And Ilima Islanders | Hilo Hula (Hilo Hanakahi) |
| 11 | DISC 1: THE SOUTHEAST | |
| 12 | –Clarence Ashley | The Coo-Coo Bird |
| 13 | –Julius Daniels | Ninety-Nine Year Blues |
| 14 | –Earl Johnson And His Clodhoppers | I Get My Whisky From Rockingham |
| 15 | DISC 5: THE DEEP SOUTH & THE WEST | |
| 16 | –Blind Willie McTell | Mamma, 'Tain't Long Fo' Day |
| 17 | –Cannon's Jug Stompers | Walk Right In |
| 18 | –Son House | My Black Mama (Parts 1 & 2) |
| 19 | –Buell Kazee | Faded Coat Of Blue |
| 20 | –Bascom Lamar Lunsford | I Wish I Was A Mole In The Ground |
| 21 | –Bumble Bee Slim And Memphis Minnie | New Orleans Stop Time |
| 22 | –The Aloha Serenaders Featuring Sol K. Bright | Tomi Tomi |
| 23 | –Weems String Band | Greenback Dollar |
| 24 | –Big Chief Henry's Indian String Band | The Indian Tom Tom |
| 25 | –Charley Patton | Down The Dirt Road Blues |
| 26 | –Dick Justice | Henry Lee |
| 27 | –Alfred G. Karnes | I Am Bound For The Promised Land |
| 28 | –Blind Willie Johnson | Dark Was The Night, Cold Was The Ground |
| 29 | –Roosevelt Graves And Brother | Woke Up This Morning (With My Mind On Jesus) |
| 30 | –Beale Street Sheiks (Stokes And Sane) | It's A Good Thing |
| 31 | –Hopi Indian Chanters | Chant Of The Eagle Dance |
| 32 | –Amédée Breaux, Ophy Breaux And Cleoma Breaux | Ma Blonde Est Partie |
| 33 | –Garfield Akers | Cottonfield Blues (Part 2) |
| 34 | –Mississippi John Hurt | Louis Collins |
| 35 | –Ernest Stoneman | The Wreck Of The '97 |
| 36 | –Mattie Delaney | Tallahatchie River Blues |
| 37 | –Henry Thomas "Ragtime Texas" | Bull Doze Blues |
| 38 | –Frank Hutchison | Stackalee |
| 39 | –Uncle Dave Macon And His Fruit Jar Drinkers | Sail Away Ladies |
| 40 | –Sol Ho'opi'i And His Novelty Trio | E, Mama Ea |
| 41 | –Robert Johnson | Cross Road Blues |
| 42 | –Geeshie Wiley | Last Kind Words Blues |
| 43 | –Nelstone's Hawaiians | Just Because |
| 44 | –Mississippi Sheiks | Sittin' On Top Of The World |
| 45 | –Ma Rainey | Prove It On Me Blues |
| 46 | –Prince Albert Hunt's Texas Ramblers | Blues In A Bottle |
| 47 | –Willie Brown | Future Blues |
| 48 | –Elder J.E. Burch | My Heart Keeps Singing |
| 49 | –Massey Family | Brown Skin Gal (Down The Lane) |
| 50 | –The Bentley Boys | Down On Penny's Farm |
| 51 | –Charlie Poole And The North Carolina Ramblers | If The River Was Whiskey |
| 52 | –William Moore | Old Country Rock |
| 53 | DISC 4: THE MIDWEST | |
| 54 | –Los Borinqueños | La Coquetera |
| 55 | –Jim Jackson | Old Dog Blue |
| 56 | –Truett And George | Ghost Dance |
| 57 | –Memphis Jug Band | On The Road Again |
| 58 | –Washington Phillips | Denomination Blues (Parts 1 & 2) |
| 59 | –Golden Melody Boys | Gonna Have 'Lasses In The Morning |
| 60 | –Jaybird Coleman | I'm Gonna Cross The River Of Jordan Some Of These Days |
| 61 | –Two Poor Boys | John Henry Blues |
| 62 | –Skip James | Cypress Grove Blues |
| 63 | –Blind Willie Davis | Trust In God And Do The Right |
| 64 | DISC 3: NEW YORK CITY | EAST COAST | |
| 65 | –Lane Hardin | Hard Time Blues |
| 66 | –Jimmie Rodgers | Waiting For A Train |
| 67 | –The Williamson Brothers And Curry | I'm Gonna Die With My Hammer In My Hand |
| 68 | –Joseph Falcon | Allons À Lafayette |
| 69 | –Sam Collins | Lonesome Road Blues |
| 70 | –Eddie Head And Family | Down On Me |
| 71 | –Tommy Johnson | Cool Drink Of Water Blues |
| 72 | –Alabama Sacred Harp Singers | Rocky Road |
| 73 | –Big Bill Broonzy | Long Tall Mama |
| 74 | DISC 2: ATLANTA | |
| 75 | –Willie Walker | Dupree Blues |
| 76 | –Richard "Rabbit" Brown | James Alley Brown |
| 77 | –Lead Belly | Mr. Tom Hughes' Town |
| 78 | –Emmett Miller | Lovesick Blues |
| 79 | –Barbecue Bob | Chocolate To The Bone |
| 80 | –Tenneva Ramblers | Darling, Where Have You Been So Long? |
| 81 | –J.P. Nester | Train On The Island |
| 82 | –Cleoma Breaux With Joseph Falcon And Ophy Breaux | Prenez Courage |
| 83 | –Blind Blake And Charlie Spand | Hastings Street |
| 84 | –Stripling Brothers | The Lost Child |
| 85 | –Andrew And Jim Baxter | K.C. Railroad Blues |
| 86 | –Guty Cárdenas Y Lencho | Coconito |
| 87 | –Delma Lachney And Blind Uncle Gaspard | La Danseuse (The Dancer) |
| 88 | –Don Richardson | Arkansas Traveler |
| 89 | –Whistler's Jug Band | Foldin' Bed |
| 90 | –Frenchy's String Band | Sunshine Special |
| 91 | –Los Madrugadores | Corrido De Joaquín Murrieta (Parts 1 & 2) |
| 92 | –Columbus Fruge | Bayou Teche |
| 93 | –Lydia Mendoza | Mal Hombre |
| 94 | –Blind Gary Davis | I Am The Light Of The World |
| 95 | –The Carter Family | Bury Me Under The Weeping Willow |
| 96 | –Banjo Joe | Poor Boy, Long Ways From Home |
| 97 | –Rev. F.W. McGee | Fifty Miles Of Elbow Room |
| 98 | –Rev. J. M. Gates | Death's Black Train Is Coming |
| 99 | –Carolina Tar Heels | Peg And Awl |
| 100 | –Berthmost Montet And Joswell Dupuis | Je Me Suis En Alle |
| 101 | –Cuarteto Flores | Cecilia |
| 102 | –Robert Wilkins | Old Jim Kinnane's |
| 103 | –Dilly And His Dill Pickles | Pickin' Off Peanuts |
| 104 | –Uncle Bunt Stephens | Sail Away Lady |
| 105 | –Blind Lemon Jefferson | See That My Grave Is Kept Clean |
(p)&(c)2017 Sony Music Entertainment